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Site directed biotinylation of filamentous phage structural proteins

Filamentous bacteriophages have been used in numerous applications for the display of antibodies and random peptide libraries. Here we describe the introduction of a 13 amino acid sequence LASIFEAQKIEWR (designated BT, which is biotinylated in vivo by E. coli) into the N termini of four of the five...

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Bibliografiske detaljer
Main Authors: Smelyanski, Larisa, Gershoni, Jonathan M
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3256129/
https://ncbi.nlm.nih.gov/pubmed/22044460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-8-495
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