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Resurrection of a Clinical Antibody: Template ProteoGenomic de novo Proteomic Sequencing and Reverse Engineering of an Anti-Lymphotoxin Alpha Antibody
A mouse hybridoma antibody directed against a member of the TNF-superfamily, lymphotoxin alpha (LT-α), was isolated from stored mouse ascites and purified to homogeneity. After more than a decade of storage the genetic material was not available for cloning, however biochemical assays with the ascit...
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| Main Authors: | , , , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3253889/ https://ncbi.nlm.nih.gov/pubmed/21268269 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201000487 |
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