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Resurrection of a Clinical Antibody: Template ProteoGenomic de novo Proteomic Sequencing and Reverse Engineering of an Anti-Lymphotoxin Alpha Antibody

A mouse hybridoma antibody directed against a member of the TNF-superfamily, lymphotoxin alpha (LT-α), was isolated from stored mouse ascites and purified to homogeneity. After more than a decade of storage the genetic material was not available for cloning, however biochemical assays with the ascit...

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Main Authors: Castellana, Natalie E., McCutcheon, Krista, Pham, Victoria C., Harden, Kristin, Nguyen, Allen, Young, Judy, Adams, Camellia, Schroeder, Kurt, Arnott, David, Bafna, Vineet, Grogan, Jane L., Lill, Jennie R.
格式: Artigo
語言:Inglês
出版: 2011
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3253889/
https://ncbi.nlm.nih.gov/pubmed/21268269
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201000487
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