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High efficiency and quantitatively reproducible protein digestion by trypsin-immobilized magnetic microspheres
Aldehyde- and NHS-activated magnetic microspheres were used to immobilize trypsin (CHO-trypsin and NHS-trypsin), and their performance for protein digestion was evaluated by reversed phase liquid chromatography-electrospray ionization-tandem mass spectrometry using an LTQ Orbitrap Velos instrument....
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3253205/ https://ncbi.nlm.nih.gov/pubmed/22176736 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2011.11.050 |
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