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Binding and cleavage of DNA with the restriction enzyme EcoR1 using time-resolved second harmonic generation
The binding of EcoR1 to a 90-bp DNA duplex attached to colloidal microparticles and the subsequent cleavage by the enzyme was observed in real time and label-free with time-resolved second harmonic (SH) spectroscopy. This method provides a unique way to investigate biomolecular interactions based on...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3250171/ https://ncbi.nlm.nih.gov/pubmed/22114185 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1115498108 |
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