Studies of Binding of Tumor Necrosis Factor (TNF)-like Weak Inducer of Apoptosis (TWEAK) to Fibroblast Growth Factor Inducible 14 (Fn14)

To perform highly sensitive cellular binding studies with TNF-like weak inducer of apoptosis (TWEAK), we developed a bioluminescent variant of soluble TWEAK (GpL-FLAG-TNC-TWEAK) by fusing it genetically to the C terminus of the luciferase of Gaussia princeps (GpL). Equilibrium binding studies on hum...

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Detalhes bibliográficos
Main Authors: Fick, Andrea, Lang, Isabell, Schäfer, Viktoria, Seher, Axel, Trebing, Johannes, Weisenberger, Daniela, Wajant, Harald
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2012
Assuntos:
Signal Transduction
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3249102/
https://ncbi.nlm.nih.gov/pubmed/22081603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.287656
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