Site-specific isotope labeling of long RNA for structural and mechanistic studies

A site-specific isotope labeling technique of long RNA molecules was established. This technique is comprised of two simple enzymatic reactions, namely a guanosine transfer reaction of group I self-splicing introns and a ligation with T4 DNA ligase. The trans-acting group I self-splicing intron with...

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Detalhes bibliográficos
Main Authors: Kawahara, Ikumi, Haruta, Kaichiro, Ashihara, Yuta, Yamanaka, Daichi, Kuriyama, Mituhiro, Toki, Naoko, Kondo, Yoshinori, Teruya, Kenta, Ishikawa, Junya, Furuta, Hiroyuki, Ikawa, Yoshiya, Kojima, Chojiro, Tanaka, Yoshiyuki
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2012
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3245953/
https://ncbi.nlm.nih.gov/pubmed/22080547
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr951
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