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Chromosome transfer in mature oocytes
In this article, we describe detailed protocols for the isolation and transfer of spindle–chromosomal complexes between mature, metaphase II-arrested oocytes. In brief, the spindle–chromosomal complex is visualized using a polarized microscope and extracted into a membrane-enclosed karyoplast. Chrom...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3244345/ https://ncbi.nlm.nih.gov/pubmed/20539289 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.75 |
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