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DNA wrapping in nucleosomes. The linking number problem re-examined.
Chromatin was assembled in vitro from relaxed closed circular DNA (SV40) and core histones at histone to DNA ratios of 0.2 to 0.3 (g/g) and incubated with topoisomerase I to relax supercoils in DNA regions not constrained by protein. Addition of histones H1 + H5 to the chromatin at an ionic strength...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1980
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC324389/ https://ncbi.nlm.nih.gov/pubmed/6255433 |
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