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Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.

Preparation of the three hexadecanucleotides, dGpTpApTpCpApCpGpApGpGpCpCpCpTpT, dCpGpApCpGpApGpCpGpTpGpApCpApCpC and cTpGpCpCpGpGpCpCpApCpGpApTpGpCpG, is described by a rapid and simple solid-phase method on polyacrylamide supports. The synthesis were performed by the extension of the method describ...

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Detalhes bibliográficos
Main Authors: Miyoshi, K, Huang, T, Itakura, K
Formato: Artigo
Idioma:Inglês
Publicado em: 1980
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC324316/
https://ncbi.nlm.nih.gov/pubmed/7465419
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