The 5'-cytosine in CCGG1 is methylated in two eukaryotic DNAs and Msp I is sensitive to methylation at this site.
Novikoff rat hepatoma and bovine liver DNAs were digested with Msp I or Hpa II. Restriction fragments were end-labeled using [alpha-32P]-dCTP and the Klenow fragment of E. coli DNA polymerase I and then digested to 2'-deoxyribonucleoside-3'-monophosphates using micrococcal nuclease and spleen phosph...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autor principal: | |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1980
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC324197/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6255419/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.17.3829 |
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