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A rapid enzymatic DNA sequencing technique: determination of sequence alterations in early simian virus 40 temperature sensitive and deletion mutants.
We have adapted a rapid sequencing technique from the enzymatic nick-translation method of Maat and Smith. The Forward-Backward procedure employs both synthetic and 3' to 5' exonucleolytic activities of E. coli DNA polymerase I to achieve greater reliability, especially in reading stretche...
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| Publié dans: | Nucleic Acids Res |
|---|---|
| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1980
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| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC324074/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6253881/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.10.2225 |
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