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A rapid enzymatic DNA sequencing technique: determination of sequence alterations in early simian virus 40 temperature sensitive and deletion mutants.

We have adapted a rapid sequencing technique from the enzymatic nick-translation method of Maat and Smith. The Forward-Backward procedure employs both synthetic and 3' to 5' exonucleolytic activities of E. coli DNA polymerase I to achieve greater reliability, especially in reading stretche...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Nucleic Acids Res
Päätekijät: Seif, I, Khoury, G, Dhar, R
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Oxford University Press 1980
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC324074/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6253881/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.10.2225
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