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A rapid enzymatic DNA sequencing technique: determination of sequence alterations in early simian virus 40 temperature sensitive and deletion mutants.

We have adapted a rapid sequencing technique from the enzymatic nick-translation method of Maat and Smith. The Forward-Backward procedure employs both synthetic and 3' to 5' exonucleolytic activities of E. coli DNA polymerase I to achieve greater reliability, especially in reading stretche...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Seif, I, Khoury, G, Dhar, R
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1980
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC324074/
https://ncbi.nlm.nih.gov/pubmed/6253881
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