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The early melting of closed duplex DNA: analysis by banding in buoyant neutral rubidium trichloroacetate.

Aqueous RbTCA permits the buoyant banding of both native and denatured DNA at room temperature and neutral pH. A unique property of this solvent is the bouyant resolution of closed circular, underwound DNA (I) from the corresponding nicked (II) species. Conditions are reported here in which PM-2 DNA...

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Autors principals: Burke, R L, Bauer, W R
Format: Artigo
Idioma:Inglês
Publicat: 1980
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC323979/
https://ncbi.nlm.nih.gov/pubmed/7443544
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