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The early melting of closed duplex DNA: analysis by banding in buoyant neutral rubidium trichloroacetate.
Aqueous RbTCA permits the buoyant banding of both native and denatured DNA at room temperature and neutral pH. A unique property of this solvent is the bouyant resolution of closed circular, underwound DNA (I) from the corresponding nicked (II) species. Conditions are reported here in which PM-2 DNA...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1980
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC323979/ https://ncbi.nlm.nih.gov/pubmed/7443544 |
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