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Purification and properties of the mini-F plasmid-encoded E protein needed for autonomous replication control of the plasmid.
Mini-F plasmid encodes a protein, E protein, that is indispensable for its autonomous replication. We have constructed a plasmid that overproduces the E protein and have purified the protein to apparent homogeneity. Using nitrocellulose filter binding and nuclease digestion assays, we demonstrated t...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1986
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323680/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3520564/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.12.4109 |
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