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Purification and properties of the mini-F plasmid-encoded E protein needed for autonomous replication control of the plasmid.

Mini-F plasmid encodes a protein, E protein, that is indispensable for its autonomous replication. We have constructed a plasmid that overproduces the E protein and have purified the protein to apparent homogeneity. Using nitrocellulose filter binding and nuclease digestion assays, we demonstrated t...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Tokino, T, Murotsu, T, Matsubara, K
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1986
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323680/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3520564/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.12.4109
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