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Cell-Free Protein Expression under Macromolecular Crowding Conditions

BACKGROUND: Cell-free protein expression (CFPE) comprised of in vitro transcription and translation is currently manipulated in relatively dilute solutions, in which the macromolecular crowding effects present in living cells are largely ignored. This may not only affect the efficiency of protein sy...

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Detalhes bibliográficos
Main Authors: Ge, Xumeng, Luo, Dan, Xu, Jianfeng
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3234285/
https://ncbi.nlm.nih.gov/pubmed/22174874
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0028707
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