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Influences of mRNA secondary structure on initiation by eukaryotic ribosomes.
Oligonucleotides designed to create hairpin structures were inserted upstream from the ATG initiator codon in several plasmids that encode preproinsulin, and the effects on translation were monitored in COS cells transfected by the vectors. Creation of a hairpin (delta G = -30 kcal/mol) that directl...
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| Autore principale: | |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
1986
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC323404/ https://ncbi.nlm.nih.gov/pubmed/3458245 |
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