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Fractionation and characterization of a yeast mRNA splicing extract.

We have fractionated a yeast whole cell extract that can accurately splice synthetic actin and CYH2 pre-mRNAs. Three fractions, designated I, II, and III, have been separated by use of ammonium sulfate fractionation and chromatography on heparin agarose. Each fraction alone has no splicing activity....

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Bibliografische gegevens
Gepubliceerd in:Proc Natl Acad Sci U S A
Hoofdauteurs: Cheng, S C, Abelson, J
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 1986
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Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323302/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3517868/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.8.2387
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