Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.

Plasmids containing the entire cDNA sequence of poliovirus type 1 (Mahoney strain) under control of a promoter for T7 RNA polymerase have been constructed. Purified T7 RNA polymerase efficiently transcribes the entire poliovirus cDNA in either direction to produce full-length poliovirus RNA [(+)RNA]...

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Udgivet i:Proc Natl Acad Sci U S A
Main Authors: van der Werf, S, Bradley, J, Wimmer, E, Studier, F W, Dunn, J J
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1986
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323290/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3010307/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.8.2330
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