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Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.
Plasmids containing the entire cDNA sequence of poliovirus type 1 (Mahoney strain) under control of a promoter for T7 RNA polymerase have been constructed. Purified T7 RNA polymerase efficiently transcribes the entire poliovirus cDNA in either direction to produce full-length poliovirus RNA [(+)RNA]...
Gardado en:
| Publicado en: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
1986
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323290/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3010307/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.8.2330 |
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