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Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Escherichia coli in complex with the acceptor-TΨC domain of tRNA

Peptidyl-tRNA hydrolase (Pth) cleaves the ester bond between the peptide and the tRNA of peptidyl-tRNA molecules, which are the product of aborted translation. In the present work, Pth from Escherichia coli was crystallized with the acceptor-TΨC domain of tRNA using 1,4-butanediol as a precipitant....

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Detalhes bibliográficos
Main Authors: Ito, Kosuke, Qi, Hao, Shimizu, Yoshihiro, Murakami, Ryo, Miura, Kin-ichiro, Ueda, Takuya, Uchiumi, Toshio
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2011
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3232141/
https://ncbi.nlm.nih.gov/pubmed/22139168
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111038383
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