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A chimeric plasmid from cDNA clones of poliovirus and coxsackievirus produces a recombinant virus that is temperature-sensitive.

We have inserted a 405-nucleotide fragment from the 5' noncoding region of the coxsackievirus B3 genome into an infectious cDNA copy of the poliovirus RNA genome. Transfection of plasmid DNA containing this hybrid genome construct into cultured monkey cells produced infectious virus. Recombinant vir...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Semler, B L, Johnson, V H, Tracy, S
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1986
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323167/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3006071/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.6.1777
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