Gyration is required for 5S RNA transcription from a chromatin template.
We have assembled transcriptionally active chromatin on 5S DNA plasmids by using a Xenopus oocyte supernatant and the 5S-specific transcription factor IIIA (TFIIIA). In this system, the 5S RNA gene is accurately transcribed at a rapid rate of 50 transcripts per gene per hr. By following the time cou...
Enregistré dans:
| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1986
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323064/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3006044/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.5.1305 |
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