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Gyration is required for 5S RNA transcription from a chromatin template.

We have assembled transcriptionally active chromatin on 5S DNA plasmids by using a Xenopus oocyte supernatant and the 5S-specific transcription factor IIIA (TFIIIA). In this system, the 5S RNA gene is accurately transcribed at a rapid rate of 50 transcripts per gene per hr. By following the time cou...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Kmiec, E B, Ryoji, M, Worcel, A
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1986
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323064/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3006044/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.5.1305
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