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Experience of islet isolation without neutral protease supplementation

We have reported improved islet isolation outcomes using a new digestion protocol where the pancreas is perfused only with collagenase, and neutral protease (NP) is administered during the digestion phase. Since the inception of this protocol, we have had some cases where administration of NP was no...

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Bibliografski detalji
Glavni autori: Kin, Tatsuya, O'Gorman, Doug, Senior, Peter, Shapiro, AM James
Format: Artigo
Jezik:Inglês
Izdano: Landes Bioscience 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3230559/
https://ncbi.nlm.nih.gov/pubmed/21099325
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/isl.2.5.12602
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