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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

MicroRNAs (miRNAs) are important regulators of gene expression and play a role in many biological processes. More than 700 human miRNAs have been identified so far with each having up to hundreds of unique target mRNAs. Computational tools, expression and proteomics assays, and chromatin-immunopreci...

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Detalhes bibliográficos
Main Authors: Aldred, Shelley Force, Collins, Patrick, Trinklein, Nathan
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3230198/
https://ncbi.nlm.nih.gov/pubmed/21988797
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3343
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