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Construction of short tandem target mimic (STTM) to block the functions of plant and animal microRNAs

Small RNAs are widespread in plants and animals. They largely include microRNAs (miRNAs) and short interfering RNAs (siRNAs), and they play key roles in gene and chromatin regulations. Here we describe in detail the method for an effective construction of the recently developed short tandem target m...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Tang, Guiliang, Yan, Jun, Gu, Yiyou, Qiao, Mengmeng, Fan, Ruiwen, Mao, Yiping, Tang, Xiaoqing
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3631596/
https://ncbi.nlm.nih.gov/pubmed/23098881
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2012.10.006
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