Bacteriophage T4 DNA topoisomerase mediates illegitimate recombination in vitro.
We have found that purified T4 DNA topoisomerase promotes recombination between two phage lambda DNA molecules in an in vitro system. In this cross, T4 DNA topoisomerase alone is able to catalyze the recombination and produce a linear monomer recombinant DNA that can be packaged in vitro. ATP is not...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Hovedforfatter: | |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1986
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322982/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3006033/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.4.922 |
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