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Purification and assay of a 145-kDa protein (STOP145) with microtubule-stabilizing and motility behavior.

The capacity of microtubules to disassemble in vitro is profoundly affected by a protein factor designated STOP (stable tubule only polypeptide). Here we report the isolation of STOP protein and confirm that its activity is, as predicted, highly substoichiometric to the tubulin in microtubules. The...

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Publicat a:Proc Natl Acad Sci U S A
Autors principals: Margolis, R L, Rauch, C T, Job, D
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 1986
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Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322919/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3456161/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.3.639
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