Detection of single base-pair mismatches in DNA by chemical modification followed by electrophoresis in 15% polyacrylamide gel.

We have developed a method for distinguishing fragments of DNA that contain single-base mismatches from their perfectly paired homologues. Single-stranded regions within a duplex fragment are accessible to 1-cyclohexyl-3-(2-[4-(4-methyl)morpholinyl]ethyl)carbodiimide, which reacts with unpaired guan...

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Detalles Bibliográficos

Publicado en:	Proc Natl Acad Sci U S A
Principais autores:	Novack, D F, Casna, N J, Fischer, S G, Ford, J P
Formato:	Artigo
Idioma:	Inglês
Publicado:	National Academy of Sciences 1986
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322908/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3003741/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.3.586
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