Detection of single base-pair mismatches in DNA by chemical modification followed by electrophoresis in 15% polyacrylamide gel.

We have developed a method for distinguishing fragments of DNA that contain single-base mismatches from their perfectly paired homologues. Single-stranded regions within a duplex fragment are accessible to 1-cyclohexyl-3-(2-[4-(4-methyl)morpholinyl]ethyl)carbodiimide, which reacts with unpaired guan...

Deskribapen osoa

Xehetasun bibliografikoak

Argitaratua izan da:	Proc Natl Acad Sci U S A
Egile Nagusiak:	Novack, D F, Casna, N J, Fischer, S G, Ford, J P
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	National Academy of Sciences 1986
Gaiak:	Research Article
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322908/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3003741/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.3.586
Etiketak:	Etiketa erantsi Etiketarik gabe, Izan zaitez lehena erregistro honi etiketa jartzen!