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Detection of foot-and-mouth disease virus rna by reverse transcription loop-mediated isothermal amplification

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64°C by employing a set of four primers targeting FMDV 2B. The assay showed higher se...

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Bibliographic Details
Main Authors: Chen, Hao-tai, Zhang, Jie, Liu, Yong-sheng, Liu, Xiang-tao
Format: Artigo
Language:Inglês
Published: BioMed Central 2011
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3222687/
https://ncbi.nlm.nih.gov/pubmed/22070774
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-8-510
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