Reverse transcription loop-mediated isothermal amplification for the rapid detection of infectious bronchitis virus in infected chicken tissues

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10(1) 50% egg infection dose (EID(50)) per 50 μl of titrated viruses a...

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Bibliographische Detailangaben
Veröffentlicht in:Mol Cell Probes
Hauptverfasser: Chen, Hao-tai, Zhang, Jie, Ma, Yan-ping, Ma, Li-na, Ding, Yao-zhong, Liu, Xiang-tao, Cai, Xue-peng, Ma, Li-qing, Zhang, Yong-guang, Liu, Yong-sheng
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier Ltd. 2010
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7126806/
https://ncbi.nlm.nih.gov/pubmed/19835950
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2009.10.001
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