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EcoK selection vectors for shotgun cloning into M13 and deletion mutagenesis.
For shotgun cloning into M13 vectors, a double-stranded cassette of synthetic oligonucleotides containing a SmaI site within the two halves of an EcoK site, has been introduced into the vector M13mp8. Cloning of blunt end DNA into the SmaI site destroys the EcoK site, and recombinants are therefore...
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| Main Authors: | , , , |
|---|---|
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1985
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| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC322152/ https://ncbi.nlm.nih.gov/pubmed/3001643 |
| Oznake: |
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