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Detection of sub-picogram quantities of specific DNA sequences on blot hybridization with biotinylated probes.

A sensitive method for detecting biotinylated DNA probes on dot and Southern blots is described which is based on the principle outlined by Leary et al (1). This system has two main components: detection of biotinylated DNA by a two-step procedure with streptavidin and poly(alkaline phosphatase); an...

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Sonraí bibleagrafaíochta
Foilsithe in:Nucleic Acids Res
Príomhchruthaitheoirí: Chan, V T, Fleming, K A, McGee, J O
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: Oxford University Press 1985
Rochtain ar líne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322111/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2933635/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.22.8083
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