Detection of sub-picogram quantities of specific DNA sequences on blot hybridization with biotinylated probes.
A sensitive method for detecting biotinylated DNA probes on dot and Southern blots is described which is based on the principle outlined by Leary et al (1). This system has two main components: detection of biotinylated DNA by a two-step procedure with streptavidin and poly(alkaline phosphatase); an...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1985
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322111/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2933635/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.22.8083 |
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