A sensitive assay for ABCA1-mediated cholesterol efflux using BODIPY-cholesterol

Studies have shown a negative association between cellular cholesterol efflux and coronary artery disease (CAD). Standard protocol for quantitating cholesterol efflux involves labeling cells with [(3)H]cholesterol and measuring release of the labeled sterol. Using [(3)H]cholesterol is not ideal for...

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Main Authors: Sankaranarayanan, Sandhya, Kellner-Weibel, Ginny, de la Llera-Moya, Margarita, Phillips, Michael C., Asztalos, Bela F., Bittman, Robert, Rothblat, George H.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2011
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3220299/
https://ncbi.nlm.nih.gov/pubmed/21957199
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D018051
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