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Rapid refinement of crystallographic protein construct definition employing enhanced hydrogen/deuterium exchange MS

Crystallographic efforts often fail to produce suitably diffracting protein crystals. Unstructured regions of proteins play an important role in this problem and considerable advantage can be gained in removing them. We have developed a number of enhancements to amide hydrogen/high-throughput and hi...

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Main Authors: Pantazatos, Dennis, Kim, Jack S., Klock, Heath E., Stevens, Raymond C., Wilson, Ian A., Lesley, Scott A., Woods, Virgil L.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2004
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC321753/
https://ncbi.nlm.nih.gov/pubmed/14715906
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0307204101
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