Development of a Real-Time Reverse-Transcription PCR for Detection of Newcastle Disease Virus RNA in Clinical Samples

A real-time reverse-transcription PCR (RRT-PCR) was developed to detect avian paramyxovirus 1 (APMV-1) RNA, also referred to as Newcastle disease virus (NDV), in clinical samples from birds. The assay uses a single-tube protocol with fluorogenic hydrolysis probes. Oligonucleotide primers and probes...

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Détails bibliographiques
Auteurs principaux: Wise, Mark G., Suarez, David L., Seal, Bruce S., Pedersen, Janice C., Senne, Dennis A., King, Daniel J., Kapczynski, Darrell R., Spackman, Erica
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2004
Sujets:
Clinical Veterinary Microbiology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC321685/
https://ncbi.nlm.nih.gov/pubmed/14715773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.1.329-338.2004
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