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Development of an Internal Positive Control for Rapid Diagnosis of Avian Influenza Virus Infections by Real-Time Reverse Transcription-PCR with Lyophilized Reagents

We developed an internal positive control (IPC) RNA to help ensure the accuracy of the detection of avian influenza virus (AIV) RNA by reverse transcription (RT)-PCR and real-time RT-PCR (RRT-PCR). The IPC was designed to have the same binding sites for the forward and reverse primers of the AIV mat...

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Detalhes bibliográficos
Main Authors: Das, Amaresh, Spackman, Erica, Senne, Dennis, Pedersen, Jan, Suarez, David L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1594697/
https://ncbi.nlm.nih.gov/pubmed/16954228
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00639-06
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