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Theoretical Design and Analysis of Multivolume Digital Assays with Wide Dynamic Range Validated Experimentally with Microfluidic Digital PCR

This paper presents a protocol using theoretical methods and free software to design and analyze multivolume digital PCR (MV digital PCR) devices; the theory and software are also applicable to design and analysis of dilution series in digital PCR. MV digital PCR minimizes the total number of wells...

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Main Authors: Kreutz, Jason E., Munson, Todd, Huynh, Toan, Shen, Feng, Du, Wenbin, Ismagilov, Rustem F.
Format: Artigo
Jezik:Inglês
Izdano: 2011
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3216679/
https://ncbi.nlm.nih.gov/pubmed/21981344
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac201658s
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