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Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae
We describe the development and characterization of a system that allows the rapid and specific induction of individual genes in the yeast Saccharomyces cerevisiae without changes in nutrients or temperature. The system is based on the chimeric transcriptional activator Gal4dbd.ER.VP16 (GEV). Upon a...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The American Society for Cell Biology
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3216669/ https://ncbi.nlm.nih.gov/pubmed/21965290 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E11-05-0466 |
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