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Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage φBT1 integrase, which belongs to the large serine recombinase subfamily. This one-step approach was efficient and...
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| Principais autores: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3216622/ https://ncbi.nlm.nih.gov/pubmed/22355658 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep00141 |
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