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Bisulfite Sequencing of DNA

Exact positions of 5-methylcytosine (m(5)C) on a single strand of DNA can be determined by bisulfite genomic sequencing (BGS). Treatment with bisulfite ion preferentially deaminates unmethylated cytosines, which then convert to uracil upon desulfonation. Amplifying regions of interest from deaminate...

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Detalhes bibliográficos
Main Authors: Darst, Russell P., Pardo, Carolina E., Ai, Lingbao, Brown, Kevin D., Kladde, Michael P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3214597/
https://ncbi.nlm.nih.gov/pubmed/20583099
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142727.mb0709s91
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