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An automated cell-counting algorithm for fluorescently-stained cells in migration assays

A cell-counting algorithm, developed in Matlab(®), was created to efficiently count migrated fluorescently-stained cells on membranes from migration assays. At each concentration of cells used (10,000, and 100,000 cells), images were acquired at 2.5 ×, 5 ×, and 10 × objective magnifications. Automat...

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Detalhes bibliográficos
Main Authors: Al-Khazraji, Baraa K, Medeiros, Philip J, Novielli, Nicole M, Jackson, Dwayne N
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3214125/
https://ncbi.nlm.nih.gov/pubmed/22011343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1480-9222-13-9
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