Construction and Characterization of Single-Chain Variable Fragment Antibody Library Derived from Germline Rearranged Immunoglobulin Variable Genes

Antibody repertoires for library construction are conventionally harvested from mRNAs of immune cells. To examine whether germline rearranged immunoglobulin (Ig) variable region genes could be used as source of antibody repertoire, an immunized phage-displayed scFv library was prepared using splenoc...

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Autors principals: Cheng, Man, Chan, Shirley Y. W., Zhao, Qi, Chan, Elaine Y. M., Au, Shannon W. N., Lee, Susanna S. T., Cheung, Wing-Tai
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2011
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3214059/
https://ncbi.nlm.nih.gov/pubmed/22096568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0027406
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