Construction and Characterization of Single-Chain Variable Fragment Antibody Library Derived from Germline Rearranged Immunoglobulin Variable Genes

Antibody repertoires for library construction are conventionally harvested from mRNAs of immune cells. To examine whether germline rearranged immunoglobulin (Ig) variable region genes could be used as source of antibody repertoire, an immunized phage-displayed scFv library was prepared using splenoc...

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Main Authors: Cheng, Man, Chan, Shirley Y. W., Zhao, Qi, Chan, Elaine Y. M., Au, Shannon W. N., Lee, Susanna S. T., Cheung, Wing-Tai
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3214059/
https://ncbi.nlm.nih.gov/pubmed/22096568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0027406
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