Directed Evolution of Pyruvate Decarboxylase-Negative Saccharomyces cerevisiae, Yielding a C(2)-Independent, Glucose-Tolerant, and Pyruvate-Hyperproducing Yeast

The absence of alcoholic fermentation makes pyruvate decarboxylase-negative (Pdc(−)) strains of Saccharomyces cerevisiae an interesting platform for further metabolic engineering of central metabolism. However, Pdc(−) S. cerevisiae strains have two growth defects: (i) growth on synthetic medium in g...

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Main Authors: van Maris, Antonius J. A., Geertman, Jan-Maarten A., Vermeulen, Alexander, Groothuizen, Matthijs K., Winkler, Aaron A., Piper, Matthew D. W., van Dijken, Johannes P., Pronk, Jack T.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2004
Fag:
Physiology and Biotechnology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC321313/
https://ncbi.nlm.nih.gov/pubmed/14711638
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.1.159-166.2004
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