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Automated sequence- and stereo-specific assignment of methyl-labeled proteins by paramagnetic relaxation and methyl–methyl nuclear overhauser enhancement spectroscopy

Methyl-transverse relaxation optimized spectroscopy is rapidly becoming the preferred NMR technique for probing structure and dynamics of very large proteins up to ~1 MDa in molecular size. Data interpretation, however, necessitates assignment of methyl groups which still presents a very challenging...

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Autors principals: Venditti, Vincenzo, Fawzi, Nicolas L., Clore, G. Marius
Format: Artigo
Idioma:Inglês
Publicat: 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3212433/
https://ncbi.nlm.nih.gov/pubmed/21935714
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-011-9559-4
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